| 產(chǎn)品編號 | bsm-61144R |
| 英文名稱 | SUMO1 Recombinant Rabbit mAb |
| 中文名稱 | 類泛素蛋白1重組兔單抗 |
| 別 名 | DAP-1; GMP1; OFC10; PIC1; SENP2; SMT3; SMT3C; SMT3H3; SUMO1; UBL1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 5F3 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:50-1:200,Flow-Cyt=1:20-1:100,ICC/IF=1:50-1:200,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 12 kDa |
| 檢測分子量 | 12 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Sumo 1: 70-101 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
SUMO1 Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. SWISS: P63165 Gene ID: 7341 合成與降解（Synthesis and Degradation） SUMO化能夠使蛋白質(zhì)更加穩(wěn)定,SUMO的生化反應(yīng)途徑與泛素相似,但不像泛素那樣誘導(dǎo)底物蛋白降解.進而調(diào)節(jié)許多關(guān)鍵的細胞活動. SUMO(small ubiquitin-related modifier)是泛素(ubiqutin)類蛋白家族的重要成員之一. |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with SUMO1 monoclonal antibody, unconjugated (bsm-61144R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed HeLa(treated with MG-132 (10μM, 1hr) ）(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (SUMO1) monoclonal Antibody, unconjugated (bsm-61144R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
