| 產(chǎn)品編號(hào) | bsm-61175R |
| 英文名稱 | DARPP32 Recombinant Rabbit mAb |
| 中文名稱 | 多巴胺/cAMP調(diào)節(jié)神經(jīng)磷蛋白重組兔單抗 |
| 別 名 | DARPP32; PPP1R1B; PPR1B; Protein phosphatase 1 regulatory subunit 1B. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | 3G6 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 23 kDa |
| 檢測分子量 | 32 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human DARPP 32: 1-54 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. SWISS: Q9UD71 Gene ID: 84152 ????DARPP-32通過自身不同位點(diǎn)的磷酸化對(duì)蛋白質(zhì)磷酸化和去磷酸化過程發(fā)揮著雙向調(diào)控的作用.DARRP-32在腦內(nèi)存在于接受多巴胺能投射的神經(jīng)元中,其磷酸化過程受多種神經(jīng)遞質(zhì)的調(diào)控,在信號(hào)轉(zhuǎn)導(dǎo)途徑中處于中心位置,整合多種胞內(nèi)信號(hào)轉(zhuǎn)導(dǎo),調(diào)節(jié)神經(jīng)元的電學(xué)和化學(xué)性質(zhì)以及動(dòng)物的生理行為反應(yīng),參與多種生理功能和病理過程,包括藥物成癮、抑郁癥、精神分裂癥等.此外,DARPP-32在非神經(jīng)系統(tǒng)的功能也逐漸被人們所認(rèn)識(shí). ????多巴胺和cAMP調(diào)節(jié)的磷蛋白(dopamine and adenosine 3 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with DARPP32 monoclonal antibody, unconjugated (bsm-61175R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DARPP32 Monoclonal Antibody, Unconjugated (bsm-61175R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DARPP32 Monoclonal Antibody, Unconjugated (bsm-61175R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Eye; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DARPP32 Monoclonal Antibody, Unconjugated (bsm-61175R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DARPP32 Monoclonal Antibody, Unconjugated (bsm-61175R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DARPP32 Monoclonal Antibody, Unconjugated (bsm-61175R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DARPP32 Monoclonal Antibody, Unconjugated (bsm-61175R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DARPP32 Monoclonal Antibody, Unconjugated (bsm-61175R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DARPP32 Monoclonal Antibody, Unconjugated (bsm-61175R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DARPP32 Monoclonal Antibody, Unconjugated (bsm-61175R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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