| 產品編號 | bsm-61191R |
| 英文名稱 | BBC3/PUMA Recombinant Rabbit mAb |
| 中文名稱 | p53正向細胞凋亡調控因子重組兔單抗 |
| 別 名 | BBC3; bcl2 binding component 3; jfy1; puma; p53 up-regulated modulator of apoptosis; PUMA alpha. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:20-50,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 21 kDa |
| 檢測分子量 | 18 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human BBC3: 150-193 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis.Promotes partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53, releasing the bound p53/TP53 to induce apoptosis. SWISS: Q9BXH1 Gene ID: 27113 PUMA在由p53誘導而經歷細胞凋亡的細胞中被p53活化。在線粒體，PUMA 誘導細胞色素C的釋放和激活對細胞凋亡的快速誘導。PUMA 與Bcl2、Bcl-XL、類Bcl2-1蛋白相互作用，促使線粒體易位和BAX的多聚化。PUMA在通過依賴細胞色素C/APAF1 途徑介導p53誘導的細胞死亡中也發(fā)揮作用。 |
| 產品圖片 |
Western blot analysis of K562 cell lysate. Using BBC3/PUMA (bsm-61191R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
