| 產(chǎn)品編號(hào) | bsm-61232R |
| 英文名稱 | APOA1 Recombinant Rabbit mAb |
| 中文名稱 | 載脂蛋白A1重組兔單抗 |
| 別 名 | APOA1; MGC117399; Apo-AI; ApoA-I; APOAI; Apolipoprotein A1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | 9B13 |
| 交叉反應(yīng) | Human,Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000,IHC-P=1:50-1:200,IHC-F=1:50-1:200,IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 31 kDa |
| 檢測(cè)分子量 | 31 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Apolipoprotein A1: 13-50 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. SWISS: P02647 Gene ID: 335 APOA1是檢測(cè)高密度脂蛋白的主要形式，APOA1降低被認(rèn)為是心、腦血管疾病的危險(xiǎn)因素。 APOA1升高：常見于肝臟疾病、肝外膽道阻塞、人工透析。 APOA1降低：常見于冠心病、未控制的糖尿病、腎病綜合征、營養(yǎng)不良、活動(dòng)性肝炎、肝功能低下、動(dòng)脈粥樣硬化、高脂蛋白血癥。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with APOA1 monoclonal antibody, unconjugated (bsm-61232R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Western blot analysis of HepG2 cell lysate. Using APOA1 (bsm-61232R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with APOA1 Monoclonal Antibody, Unconjugated (bsm-61232R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with APOA1 Monoclonal Antibody, Unconjugated (bsm-61232R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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