| 產品編號 | bsm-61234R |
| 英文名稱 | CA9 Recombinant Rabbit mAb |
| 中文名稱 | 碳酸酐酶9重組兔單抗 |
| 別 名 | MN; P54/58N; CAIX; CA9; Carbonic anhydrase IX; CAH9. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 10A7 |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 50 kDa |
| 檢測分子量 | 50 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Carbonic anhydrase 9: 204-256 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions). SWISS: Q16790 Gene ID: 768 ????碳酸酐酶9（carbonic anhydrase IX，CA IX）是新發(fā)現(xiàn)的碳酸酐酶家族異構體之一，是由酸性氨基酸組成的跨膜糖蛋白，在調控細胞增殖、轉化方面有重要作用。 ????碳酸酐酶9能催化CO2水解為碳酸和水，參與機體的酸堿平衡，調節(jié)細胞內外pH值，易導致腫瘤的生長和轉移，目前也是子宮腫瘤新的標記物。 |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with CA9 monoclonal antibody, unconjugated (bsm-61234R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CA9 Monoclonal Antibody, Unconjugated(bsm-61234R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CA9 Monoclonal Antibody, Unconjugated(bsm-61234R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
