| 產(chǎn)品編號 | bsm-61247R |
| 英文名稱 | SERCA1 Recombinant Rabbit mAb |
| 中文名稱 | 肌漿網(wǎng)鈣ATP酶1/內(nèi)質(zhì)網(wǎng)鈣ATP酶1重組兔單抗 |
| 別 名 | ATP2A; ATP2A1; SERCA1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:1000-1:2000,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 110 kDa |
| 檢測分子量 | 110 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human SERCA1: 970-1001 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Key regulator of striated muscle performance by acting as the major Ca2+ ATPase responsible for the reuptake of cytosolic Ca2+ into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen.Contributes to calcium sequestration involved in muscular excitation/contraction. SWISS: O14983 Gene ID: 487 |
| 產(chǎn)品圖片 |
Western blot analysis of Human fetal muscle lysate. Using SERCA1 (bsm-61247R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed RD (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (SERCA1) monoclonal Antibody, unconjugated (bsm-61247R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
|
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
