| 產品編號 | bsm-61355R |
| 英文名稱 | phospho-CBL (Ser669) Recombinant Rabbit mAb |
| 中文名稱 | 磷酸化原癌蛋白CBL2重組兔單抗 |
| 別 名 | Casitas B lineage lymphoma proto oncogene; cbl; CBL2; E3 ubiquitin protein ligase CBL; Oncogene CBL2; Proto oncogene c CBL; RING finger protein 55; RNF55; Signal transduction protein CBL. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human (predicted: Mouse,Rat) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 100 kDa |
| 檢測分子量 | 120 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human CBL around the phosphorylation site of S669: SS-pS-AN |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. SWISS: P22681 C CBL是泛素一蛋白酶體(ubiquitin proteasome)通路中的一個新的RINGFinger型泛素連接酶(ubiquitinligase ,E3).C CBL可以介導受體酪氨酸激酶和非受體酪氨酸受體激酶的降解. |
| 產品圖片 |
Jurkat (H) cells were treated with or without pervanadate (1nM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-CBL (Ser669) monoclonal antibody, unconjugated (bsm-61355R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
