| 產(chǎn)品編號 | bsm-61422R |
| 英文名稱 | CTCF Recombinant Rabbit mAb |
| 中文名稱 | 轉(zhuǎn)錄阻抑蛋白CTCF重組兔單抗 |
| 別 名 | Ctcf; CTCFL paralog; MRD21. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:50-100,ICC/IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 83 kDa |
| 檢測分子量 | 140 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human CTCF: 680-727 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. SWISS: P49711 Gene ID: 10664 CTCF是一種結(jié)構(gòu)保守、功能多樣的鋅指蛋白。通過不同鋅指的組合，識別不同的DNA序列，并且通過自身形成多聚體或與其它蛋白相互作用、與亞細(xì)胞核結(jié)構(gòu)共定位等多種方式，在基因活化與沉默、基因印記、X-染色體失活以及基因組核內(nèi)空間組織等不同生理過程中發(fā)揮關(guān)鍵作用。CTCF在細(xì)胞生長與增殖、系統(tǒng)發(fā)生以及腫瘤發(fā)生等方面都有著重要的作用。 |
| 產(chǎn)品圖片 |
Western blot analysis of (1) HeLa cell lysate; (2) Mouse brain lysate. Using CTCF (bsm-61422R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (CTCF) monoclonal Antibody, unconjugated (bsm-61422R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-CTCF antibody (bsm-61422R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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