| 產(chǎn)品編號(hào) | bsm-61453R |
| 英文名稱 | PRMT1 Recombinant Rabbit mAb |
| 中文名稱 | 蛋白質(zhì)精氨酸甲基轉(zhuǎn)移酶1重組兔單抗 |
| 別 名 | Histone-arginine N-methyltransferase PRMT1; ANM1; HCP1; IR1B4; HRMT1L2. |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:1000-2000,IHC-P=1:100-200,IHC-F=1:100-200,IF=1:50-200,Flow-Cyt=1:50-100,ICC/IF=1:50-200,IP=1:20-50,ChIP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 42 kDa |
| 檢測(cè)分子量 | 42 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human PRMT1: 11-61 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, FMR1, ILF3, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4, SERBP1, RBM15, FOXO1, CHTOP, MAP3K5/ASK1 and NPRL2. SWISS: Q99873 Gene ID: 3276 |
| 產(chǎn)品圖片 |
Western blot analysis of (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) PC-12 cell lysate. Using PRMT1 (bsm-61453R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PRMT1) monoclonal Antibody, unconjugated (bsm-61453R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-PRMT1 antibody (bsm-61453R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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