| 產(chǎn)品編號(hào) | bsm-61460R |
| 英文名稱 | Ku70 / XRCC6 Recombinant Rabbit mAb |
| 中文名稱 | DNA修復(fù)酶Ku70重組兔單抗 |
| 別 名 | CTC75; CTCBF; DNA repair protein XRCC6; G22P1; Ku 70; Ku autoantigen 70kDa; Ku autoantigen, 70kDa; Ku p70; Ku70; Kup70; TLAA; XRCC6. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | 1C4 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1:50-100,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 70 kDa |
| 檢測(cè)分子量 | 70 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Ku70: 525-568 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA. Required for double-strand break repair and V(D)J recombination. SWISS: P12956 Gene ID: 2547 Ku-70是一種DNA修復(fù)蛋白，當(dāng)細(xì)胞在受到輻射損傷而發(fā)生DNA雙鏈斷裂時(shí)，Ku70可迅速將其修復(fù)，從而提高細(xì)胞存活率. Ku是一種多功能的蛋白，在許多重要的細(xì)胞生命過程中起著直接或間接的作用，如DNA雙鏈斷裂的修復(fù)，免疫球蛋白和T細(xì)胞受體V（D）J重排，免疫球蛋白構(gòu)型轉(zhuǎn)換，DNA復(fù)制，DNA轉(zhuǎn)錄的調(diào)節(jié)，同時(shí)在細(xì)胞周期的G2和M時(shí)相中起著特殊的作用. |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Ku70 / XRCC6 monoclonal antibody, unconjugated (bsm-61460R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ku70 / XRCC6 Monoclonal Antibody, Unconjugated (bsm-61460R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ku70 / XRCC6 Monoclonal Antibody, Unconjugated (bsm-61460R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ku70 / XRCC6 Monoclonal Antibody, Unconjugated (bsm-61460R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ku70 / XRCC6 Monoclonal Antibody, Unconjugated (bsm-61460R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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