| 產品編號 | bsm-61479R |
| 英文名稱 | Glucose 6 Phosphate Dehydrogenase Recombinant Rabbit mAb |
| 中文名稱 | 葡萄糖-6磷酸脫氫酶重組兔單抗 |
| 別 名 | G6PD; G6PD1; G6pdx; Glucose 6 phosphate 1 dehydrogenase; Glucose 6 phosphate dehydrogenase; Glucose 6 phosphate dehydrogenase, G6PD; MET19; POS10; Zwf1p. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 9E20 |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:50-100,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 59 kDa |
| 檢測分子量 | 59 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human G6PD: 460-515/515 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. SWISS: P11413 Gene ID: 2539 G-6-PD（Glucose-6-phosphate Dehydrogenase）。一種存在於人體紅血球內，協(xié)助葡萄糖進行新陳代謝之酵素，在這代謝過程中會產生一種叫NADPH的物質能以保護紅血球免受氧化物質的威脅。 G6PD缺乏時，若身體接觸到具氧化性的特定物質或服用了這類藥物，紅血球就容易被破壞而發(fā)生急性溶血反應。 |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Glucose 6 Phosphate Dehydrogenase monoclonal antibody, unconjugated (bsm-61479R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Glucose 6 Phosphate Dehydrogenase Monoclonal Antibody, Unconjugated(bsm-61479R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Glucose 6 Phosphate Dehydrogenase Monoclonal Antibody, Unconjugated(bsm-61479R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Glucose 6 Phosphate Dehydrogenase Monoclonal Antibody, Unconjugated(bsm-61479R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Glucose 6 Phosphate Dehydrogenase Monoclonal Antibody, Unconjugated(bsm-61479R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
