| 產(chǎn)品編號(hào) | bsm-61505R | 
| 英文名稱 | alpha Sarcoglycan Recombinant Rabbit mAb | 
| 中文名稱 | α肌萎縮糖蛋白2重組兔單抗 | 
| 別 名 | 50DAG; 50kD DAG; adhalin; ADL; alpha SG; alpha-sarcoglycan; Asg; DAG2; DMDA2; Dystroglycan 2; LGMD2D; SCARMD1; Sgca. | 
| 抗體來(lái)源 | Rabbit | 
| 克隆類型 | Recombinant | 
| 克 隆 號(hào) | 4A2 | 
| 交叉反應(yīng) | Human,Mouse,Rat | 
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,IP=1:20-50
 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.  | 
| 理論分子量 | 43 kDa | 
| 檢測(cè)分子量 | 50 | 
| 性 狀 | Liquid | 
| 免 疫 原 | A synthesized peptide derived from human alpha Sarcoglycan: 320-387 | 
| 亞 型 | IgG | 
| 純化方法 | affinity purified by Protein A | 
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. | 
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. | 
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. | 
| PubMed | PubMed | 
| 產(chǎn)品介紹 | 
Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. SWISS: Q16586 Gene ID: 6442  | 
| 產(chǎn)品圖片 | 
 
25 ug total protein per lane of various lysates (see on figure) probed with alpha Sarcoglycan monoclonal antibody, unconjugated (bsm-61505R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
 
Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with alpha Sarcoglycan Monoclonal Antibody, Unconjugated (bsm-61505R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
 
Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with alpha Sarcoglycan Monoclonal Antibody, Unconjugated (bsm-61505R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
 
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with alpha Sarcoglycan Monoclonal Antibody, Unconjugated (bsm-61505R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
 
Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with alpha Sarcoglycan Monoclonal Antibody, Unconjugated (bsm-61505R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
 
Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with alpha Sarcoglycan Monoclonal Antibody, Unconjugated (bsm-61505R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
 
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