| 產(chǎn)品編號 | bsm-62425R |
| 英文名稱 | RCHY1 Recombinant Rabbit mAb |
| 中文名稱 | 泛素連接酶重組兔單抗 |
| 別 名 | ARNIP; CHIMP; hARNIP; hPirh2; PIRH2E; PIRH2F; RCHY1; RNF199; ZCHY; ZNF363. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse |
| 產(chǎn)品應用 | WB=1:1000-1:2000,ICC/IF=1:50-1:200,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 30 kDa |
| 檢測分子量 | 30 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human RCHY1: 160-261 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
E3 ubiquitin-protein ligase that mediates ubiquitination of target proteins, including p53/TP53, TP73, HDAC1 and CDKN1B. SWISS: Q96PM5 Gene ID: 25898 Pirh2通過負反饋調(diào)控p53的轉(zhuǎn)錄激活和細胞生長抑制功能； Pirh2蛋白是一個新發(fā)現(xiàn)的泛素蛋白連接酶，受到p53的誘導調(diào)控表達，可促進p53蛋白的泛素化降解。目前多用于肺癌的研究。 |
| 產(chǎn)品圖片 |
Western blot analysis of 293 cell lysate. Using RCHY1 (bsm-62425R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
