| 產(chǎn)品編號 | bsm-62650R |
| 英文名稱 | TPMT Recombinant Rabbit mAb |
| 中文名稱 | 硫嘌呤甲基轉(zhuǎn)移酶重組兔單抗 |
| 別 名 | Thiopurine methyltransferase; TPMT. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:100-1:200,Flow-Cyt=1:20-1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 28 kDa |
| 檢測分子量 | 30 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human TPMT: 101-125 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine. SWISS: P51580 Gene ID: 7172 TPMT是硫嘌呤類藥物（如硫唑嘌呤、巰嘌呤和硫鳥嘌呤）的代謝中最主要的酶之一，起到的作用是在這類化合物的硫原子上增加一個甲基；這個過程中提供甲基的是S-腺苷甲硫氨酸，后者同時被轉(zhuǎn)化成S-腺苷-L-高半胱氨酸。 TPMT基因的缺陷會讓人體無法將這類藥物滅活，未代謝的藥物因此在體內(nèi)大量累積，從而引起嚴重甚至致命的骨髓抑制，表現(xiàn)為貧血、血小板減少癥（導(dǎo)致出血）和白細胞減少癥（導(dǎo)致感染）等. |
| 產(chǎn)品圖片 |
Western blot analysis of Jurkat cell lysate. Using TPMT (bsm-62650R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
