產(chǎn)品編號 | bs-1845R |
英文名稱 | LRIG2 Rabbit pAb |
中文名稱 | LRIG2抗體 |
別 名 | DKFZp451C181; KIAA0806; LIG2; leucine-rich repeats and immunoglobulin-like domains protein 2 precursor; LRIG2_HUMAN; UFS2; LIG-2. |
研究領(lǐng)域 | 腫瘤 細胞生物 免疫學 信號轉(zhuǎn)導 激酶和磷酸酶 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
克 隆 號 | |
交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Dog,GuineaPig,Horse) |
產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 117 kDa |
檢測分子量 | |
細胞定位 | 細胞膜 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human LRIG2: 701-800/1065 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
LRIG2 is produced in all human glioma cell lines and localizes to perinuclear compartments, cytoplasmic compartments and the cell surface. It acts as a negative feedback regulator of signaling through enhanced receptor ubiquitination and accelerated intracellular degradation. LRIG2 may function as a tumour suppressor since it downregulates the expression of EGF and the related proteins ErbB-2, ErbB-3 and ErbB-4, which all inhibit cancer cells from growth, migration and invasion. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Cytoplasm. Tissue Specificity: Detected in all tissues analyzed. Similarity: Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 15 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain. SWISS: O94898 Gene ID: 9860 Database links: Entrez Gene: 9860 Human Omim: 608869 Human SwissProt: O94898 Human Unigene: 448972 Human LRIG1屬于LRIGs基因家族。LRIGs是新發(fā)現(xiàn)的一類基因,在哺乳動物體內(nèi)廣泛存在,目前發(fā)現(xiàn)該家族至少包括三個成員:LRIG1、LRIG2和LRIG3,是一類結(jié)構(gòu)相似的跨膜糖蛋白,該蛋白人、鼠的同源性較高。 目前在腫瘤方面的研究較多,有研究認為:LRIG1是通過下調(diào)EGFR的表達來抑制其下游重要信號傳導通路的激活而發(fā)揮抑癌作用,LRIG1在抑制腫瘤轉(zhuǎn)移中具有重要作用。 |
產(chǎn)品圖片 |
Sample:
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti- LRIG2 (bs-1845R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 117 kD
Observed band size: 117 kD
Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human HepG2 cell lysates
Primary: Anti-LRIG2 (bs-1845R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDa
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設(shè)計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |