| 產(chǎn)品編號 | bs-5378R |
| 英文名稱 | PLCZ1 Rabbit pAb |
| 中文名稱 | 磷酸肌醇磷脂酶PLCZ1抗體 |
| 別 名 | 1 phosphatidylinositol 4, 5 bisphosphate phosphodiesterase zeta 1; phosphoinositide phospholipase C zeta 1; phospholipase C zeta 1; PI phospholipase C zeta 1; PLC zeta 1; PLCzeta; Testis development protein NYD SP27; Testis development related NYD SP27; MGC149685 antibody NYD SP27 antibody. |
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Specific References (3) | bs-5378R has been referenced in 3 publications.
[IF=6.684] Meng Wang. et al. A Novel Assisted Oocyte Activation Method Improves Fertilization in Patients With Recurrent Fertilization Failure. Front Cell Dev Biol. 2021; 9: 672081 IF ; Human.
[IF=3.322] Xiaopei Zhou. et al. Actl7a deficiency in mice leads to male infertility and fertilization failure. BIOCHEM BIOPH RES CO. 2022 Oct;623:154 IF ; Mouse.
[IF=2.98] Zhou, Xiaopei. et al. A novel homozygous mutation in ACTL7A leads to male infertility. MOL GENET GENOMICS. 2022 Dec;:1-8 IF ; Human.
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| 研究領(lǐng)域 | 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 70 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PLCZ1: 351-450/608 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, PLCZ1 hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. It triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. PLCZ1 is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. It may exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function. Tissue Specificity: Expressed specifically in testis and sperm. Weakly expressed in pancreatic-duct cells. Up-regulated in pancreatic-duct cells from patients with cystic fibrosis. SWISS: Q86YW0 Gene ID: 89869 Database links: Entrez Gene: 89869 Human Entrez Gene: 114875 Mouse Omim: 608075 Human SwissProt: Q86YW0 Human SwissProt: Q8K4D7 Mouse Unigene: 97542 Human Unigene: 50808 Mouse Unigene: 123184 Rat |
| 產(chǎn)品圖片 |
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PLCZ1 Polyclonal Antibody, Unconjugated(bs-5378R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PLCZ1) Polyclonal Antibody, Unconjugated (bs-5378R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
