| 產品編號 | bsm-63151R |
| 英文名稱 | Cytokeratin 6 Recombinant Rabbit mAb |
| 中文名稱 | 細胞角蛋白6重組兔單抗 |
| 別 名 | CK 6A; CK 6B; CK 6C; CK 6D; CK 6E; CK-6C; CK-6E; Cytokeratin 6a; Cytokeratin 6B; Cytokeratin 6C; Cytokeratin 6D; Cytokeratin 6E; Cytokeratin-6C; Cytokeratin-6E; K6a keratin; K6b keratin; K6C; K6c keratin; K6d keratin; K6e keratin; Keratin K6h. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 1B7 |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-1:2000,IHC-P=1:100-1:500,IHC-F=1:100-1:500,IF=1:100-1:500,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 60 kDa |
| 檢測分子量 | 60 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Cytokeratin 6A: 1-45 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair. SWISS: P48668 Gene ID: 3854 結構蛋白（Structural Proteins）細胞角蛋白是一類與結構相關的蛋白家族，其在上皮細胞中形成細胞骨架中間絲。CK6表達于鱗狀上皮細胞，特別是增生的鱗狀上皮。76%的腦、頸部鱗狀細胞癌病例中有CK6的表達。 |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytokeratin 6 Monoclonal Antibody, Unconjugated (bsm-63151R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytokeratin 6 Monoclonal Antibody, Unconjugated (bsm-63151R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human lung squamous cell carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytokeratin 6 Monoclonal Antibody, Unconjugated (bsm-63151R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 6 Monoclonal Antibody, Unconjugated(bsm-63151R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Abdominal skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytokeratin 6 Monoclonal Antibody, Unconjugated (bsm-63151R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
