| 產(chǎn)品編號 | bsm-63256R |
| 英文名稱 | Villin Recombinant Rabbit mAb |
| 中文名稱 | 絨毛蛋白重組兔單抗 |
| 別 名 | VIL; VIL1; Villin1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 5A12 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:100-1:200,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 93 kDa |
| 檢測分子量 | 80 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Villin 1: 640-693/827 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Epithelial cell-specific Ca2+-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. SWISS: P09327 Gene ID: 7429 絨毛蛋白(Villin)是一種分子量為93-95kDa的肌動蛋白相關(guān)鈣調(diào)節(jié)蛋白,正常分布于腸上皮和腎近曲小管上皮,可用于胃腸道神經(jīng)內(nèi)分泌腫瘤診斷的參考指標。 |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |