產(chǎn)品編號 | bs-9773R-FITC |
英文名稱 | Rabbit Anti-C10orf63/FITC Conjugated antibody |
中文名稱 | FITC標記的10號染色體開放閱讀框63抗體 |
別 名 | DKFZp781F21103; Chromosome 10 open reading frame 63; ENKUR; Enkurin; ENKUR_HUMAN. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
研究領域 | 腫瘤 細胞生物 免疫學 信號轉(zhuǎn)導 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | (predicted: Human, Mouse, Rat, Dog, Cow, Sheep, ) |
產(chǎn)品應用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 29kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human C10orf63/Enkurin |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: Enkurin is a 256 amino acid adapter protein that brings signal transduction proteins and transient receptor potential canonical (TRPC) cation channels into contact. Localizing to the acrosomal crescent and flagellar principal piece of sperm, Enkurin contains one IQ domain which it uses to bind CaM (calmodulin). Enkurin is highly expressed in testis and vomeronasal organ, but is also found at lower levels in brain, ovary, heart and lung. The gene encoding Enkurin maps to human chromosome 10, which contains over 800 genes and 135 million nucleotides. PTEN is an important tumor suppressor gene located on chromosome 10 and, when defective, causes a genetic predisposition to cancer development known as Cowden syndrome. Other chromosome 10 associated disorders include Cockayne syndrome, tetrahydrobiopterin deficiency and trisomy 10. Function: Adapter that functions to localize a calcium-sensitive signal transduction machinery in sperm to a calcium-permeable ion channel (By similarity). Subunit: Binds calmodulin via its IQ domain. Interacts with TRPC1, TRPC2, TRPC5, but not TRPC3 (By similarity). Subcellular Location: Cell projection, cilium, flagellum. Note=Sperm acrosomal crescent and flagellar principal piece. Similarity: Contains 1 IQ domain. Database links: Entrez Gene: 219670 Human Entrez Gene: 71233 Mouse GenBank: NM_145010 Human Omim: 611025 Human SwissProt: Q8TC29 Human SwissProt: Q6SP97 Mouse Unigene: 534486 Human Unigene: 27658 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |